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Sperm Counting Slide

  • Pre-Stained Morphology Slides For Sperm Morphology Analysis
Pre-Stained Morphology Slides For Sperm Morphology Analysis

Pre-Stained Morphology Slides For Sperm Morphology Analysis

  • name:Pre-Stained Morphology Slides
  • usage:sperm morphology analysis
  • apply:CASA Semen Analysis
  • Approval:CE FDA
  • Product description: Pre-Stained Morphology Slides For Sperm Morphology Analysis
  • INQUIRY

ASCEN Pre Stained Morphology Slide Intended use:


to assess the morphological parmeters of cells such as spermatozoa. The stained cells can be assessed with them help of an automated system(i.e. CASA) or manually。


Principle of the device:

when a small volume of cells / semen is deposited on a pre-stained slide,the fixative-stain film dissolves in fluid/seminal plasma and stains (sperm) cells。


Preparations before use:

Remove ASCEN pre-stained morphology slide from packaging and take care not to touch the coated area. Make sure the coated area is undamaged. Access toan incubator at 55°C / 131F, or an ethanol flame. Use semen after liquefaction or (sperm) sample in a culture medium。


Staining Procedure:


*Check the color stained on surface before add on sample 。


*Pipette 5µl of sample on the endside of the slide prepare the smear like figure 1。


*Put the slide on the hotplate 56°C 30 -60 seconds。


*Drop the slide into the water jar 1 second。


*Dry the slide in hotplate。


*Start assessment immediately after。


Automatic analyzing  -use SCA sperm morph.


System In order to improve the efficiency of the accuracy of the sperms identification


We recommend to  wash the sample .


Wash procedure


1.mix gently of 0.2~0.5ml (relate with the density of sperm) liquefaction semen with 10ml physiological saline or 10ml PBS (pH7.4 ± 0.2)


2.centrifugal 10min 800g


3.removing most of the supernatant


4.Mix gently the rest, usually a 20~40µl.


5.count the concentration of sperm


1)= 45M/ml,perfect go to Staining procedure


2)< 45M/ml,add 20ul physiological saline or 10ml PBS (pH7.4 ± 0.2) repeat step 2



3)> 45M/ml,dilute


pre stained morphology slide


pre stained morphology slides


sperm morphology slide

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